Non-standard abbreviations used: CYP: cytochrome P450; HT: hexahistidine-tagged; ES/LC-MS: electrospray ionization/liquid chromatography mass spectrometry; PMSF: phenylmethylsulfonyl fluoride; CHAPS: 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propanesulfonate; EDDP: 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine; TFA: trifluoroacetic acid

An enantiomer-selective liquid chromatography-tandem mass spectrometry method for methadone and EDDP validated for use in human plasma, urine, and liver microsomes.

A liquid chromatography-electrospray ionization-tandem mass spectrometry method has been developed and validated to detect (R)- and (S)-methadone and (R)- and (S)-2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in human plasma with cross-validation to urine and liver microsomes. Use of deuterated internal standards and liquid-liquid extraction coupled with chiral separation provided ba...

Determination and Validation of a Solid-phase Extraction Gas Chromatography-mass Spectrometry for the Quantification of Methadone and Its Principal Metabolite in Human Plasma

This study aimed to develop a solid-phase extraction gas chromatography-selected ion monitoring-mass spectrometry method for the determination of methadone (MDN) and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in human plasma. The linear response was obtained over the concentration range from 10 to 2000 ng/mL for MDN and EDDP. The absolute recoveries of MDN and EDDP were 95.9%-98.9...

Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS)

The current research aimed to quantify melittin (MEL) in Iranian honey bee (Apis mellifera meda) venom. To this end, a liquid chromatography-electrospray ionization-ion trap tandem mass spectrometry (LC-ESI-IT-MS/MS) approach was employed. Melittin is the main toxic peptide of honey bee venom with various biological and pharmacological activities. It was extracted with...

List of non-standard abbreviations: ACN acetonitrile CYP cytochrome P450 DCM dichloromethane EC electrochemistry ESI-MS electrospray ionization mass spectrometry

Sample preparation in analysis of pharmaceuticals

Abbreviations: AIBN, 2,20-azo-bis-isobutyronitrile; ASE, Accelerated solvent extraction; CAR, Carboxen; DAD, Diode-array detector; DI-SPME, Direct-immersion solid-phase extraction; DSPE, Dispersive solid-phase extraction; EGDMA, Ethylene glycol dimethacrylate; ESE, Enhanced solvent extraction; ESI, Electrospray ionization; FD, Fluorescence detection; FLD, Fluorimetric detection; GC, Gas chromat...